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KMID : 1059520100540020215
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Journal of the Korean Chemical Society
2010 Volume.54 No. 2 p.215 ~ p.221
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Detection of Salmonella Using the Loop Mediated Isothermal Amplification and Real-time PCR
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Ahn Young-Chang
Cho Min-Ho
Yoon Il-Kyu
Jung Duck-Hyun
Lee Eun-Young
Kim Jin-Ho
Jang Won-Choeul
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Abstract
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Salmonella is an important food-and water-borne pathogen associated with acute gastrointestinal illnesses around the world. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the Loop Mediated Isothermal Amplification and real-time PCR has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a LAMP and real-time PCR was used to detect S. Typhimurium and S. Enteritidis. We selected target genes, which were the in invA and a randomly cloned sequence specific for the genus Salmonella. With LAMP and real-time PCR, random sequence was detected from Salmonella spp, invA were detected from all strain of S. Typhimurium and S. Enteritidis. This assay indicate that the specificity, sensitivity and rapid of the LAMP and real-time PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.
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KEYWORD
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LAMP(Loop Mediated Isothermal Amplification), PCR(Polymerase Chain Reaction), Salmonella Typhimurium, Salmonella Enteritidis
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